cell culture hela (ATCC)

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ATCC cell culture hela
Cell Culture Hela, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 38021 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+culture+hela/293T/pm41963349-257-0-4
Average 99 stars, based on 38021 article reviews

cell culture hela - by Bioz Stars, 2026-09

99/100 stars

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Article Title: Chemical probe-dependent evaluation of protein activity and uses thereof
Article Snippet: .. Cell Culture HeLa, PC3, LNCaP, SKOV3, MCF7 and U87 and OVCAR3 cell lines were obtained from ATCC and were not STR profiled. ..

Article Title: Surfactin-based lipopeptide for mRNA delivery
Article Snippet: .. Cell Culture HeLa and HepG2 cell lines were obtained from rom the American Type Culture Collection (ATCC) (Rockville, MD, USA). ..

Article Title: Translation in proximity to forming autophagosomes during sustained autophagy.
Article Snippet: .. Cell culture HeLa (CCL2, ATCC) and HEK293T (CRL-3216, ATCC) cells were cultured in complete medium (Dulbecco’s Modified Eagle’s Medium containing 10% fetal bovine serum and 1% penicillin-streptomycin) in the incubator at 37°C with 5% CO2. ..

Article Title: Rapid selection of transgenic mammalian cells via diphtheria toxin resistance.
Article Snippet: Cell culture HeLa (ATCC Cat# CRM-CCL-2, RRID:CVCL_0030) cells were obtained from ATCC (Manassas, VA, USA) and cultivated in Dulbecco’s Modified Eagle Medium (DMEM), high glucose, GlutaMAX supplemented with 10% (v/v) fetal bovine serum (FBS) and 1% (v/v) 100 x Penicillin-Streptomycin (DMEM++) all from Thermo Fisher Scientific (Waltham, MA, USA) at 37°C and 5% CO2. .. Cell culture HeLa (ATCC Cat# CRM-CCL-2, RRID:CVCL_0030) cells were obtained from ATCC (Manassas, VA, USA) and cultivated in Dulbecco’s Modified Eagle Medium (DMEM), high glucose, GlutaMAX supplemented with 10% (v/v) fetal bovine serum (FBS) and 1% (v/v) 100 x Penicillin-Streptomycin (DMEM++) all from Thermo Fisher Scientific (Waltham, MA, USA) at 37°C and 5% CO2. .. Human embryonic kidney (HEK) FreeStyle 293-F cells ((RRID:CVCL_D603), Thermo Fisher Scientific) were cultured in 30 mL FreeStyle medium (Thermo Fisher Scientific) in a 125 mL disposable flask (Jet Bio-Filtration Co., Ltd, Guangzhou, China).

Article Title: The neuropathy-linked protein TECPR2 is a Rab5 effector that regulates cargo recycling from early endosomes.
Article Snippet: .. Cell culture HeLa and HEK293T (from ATCC) cells were cultured in DMEM media (Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Gibco) in a humidified cell culture incubator (New Brunswick) with 5% CO2 at 37 °C. hTERT-RPE1 cells (from ATCC) were cultured in DMEM/F-12 media (Gibco), supplemented with 10% FBS. .. For the livecell imaging and flow cytometry experiments, cells were cultured in phenol red-freemedia (Gibco) supplemented with 10% FBS.

Article Title: A Commander-independent function of COMMD3 in endosomal trafficking
Article Snippet: Sequence- based reagents COMMD3 CRISPR guide RNA sequence primers This paper N/A CTTC GCGC TTCT CCTC CGGG Sequence- based reagents COMMD3 CRISPR guide RNA sequence primers This paper CTTG AAAC AGAT CGAC CCAG Sequence- based reagents COMMD1 CRISPR guide RNA sequence primers This paper TCAC GGAC ACTC GGGT GTCA Sequence- based reagents Commd1 CRISPR guide RNA sequence primers This paper ACTG CTCA AACC AAAA AGCA Sequence- based reagents Commd5 CRISPR guide RNA sequence primers This paper GTTG TTGA AACT CGTA GTCG Sequence- based reagents Commd5 CRISPR guide RNA sequence primers This paper TGCC AGCG CCAA CCTG TCAG Sequence- based reagents Ccdc93 CRISPR guide RNA sequence primers This paper CGAA AGTA CCGA CGGC AGCG Sequence- based reagents Ccdc93 CRISPR guide RNA sequence primers This paper GATG ACCG CCAT GGCA AACG Sequence- based reagents Vps35l CRISPR guide RNA sequence primers This paper GGAT TATG TGAA CCGC ATAG Sequence- based reagents Vps35l CRISPR guide RNA sequence primers This paper GGAG GTTT GCAA GTGC ATCA Antibody anti- HA mouse monoclonal BioLegend #901501, RRID:AB_2565006 Flow (1:250), IF (1:1000) Antibody APC- conjugated anti- LAMP1 mouse monoclonal BioLegend #328619, RRID:AB_1279055 Flow (1:250) Antibody anti- ITGA6 rat monoclonal Invitrogen #14- 0495- 82, RRID:AB_891480 Flow (1:250) Antibody APC- conjugated anti- mouse secondary antibodies rat monoclonal eBioscience #17- 4015- 82, RRID:AB_2573205 Flow (1:1000) Antibody APC- conjugated anti- rat antibodies goat polyclonal Invitrogen #A10540, RRID:AB_10562535 Flow (1:1000) Antibody APC- conjugated anti- TfR/CD71 antibodies mouse monoclonal BioLegend #334108, RRID:AB_10915138 Flow (1:500) Antibody anti- FLAG M2 mouse monoclonal Sigma- Aldrich #F1804, RRID:AB_262044 IF (1:1000) Antibody Alexa Fluor 647- conjugated anti- rabbit IgG goat polyclonal Invitrogen #A32733, RRID:AB_2633282 IF (1:1000) Continued on next page Continued Squiers et al. eLife 2025;14:RP105264. .. DOI: https://doi.org/10.7554/eLife.105264 18 of 25 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Alexa Fluor 568- conjugated anti- mouse IgG goat polyclonal Thermo Fisher Scientific #A11004, RRID:AB_2534072 IF (1:1000) Antibody Anti- COMMD3 rabbit polyclonal Bethyl #A304- 092A, RRID:AB_2621341 IB (1:200) Antibody anti- VPS35L rabbit polyclonal Invitrogen #PA5- 28553, RRID:AB_2546029 IB (1:500) Antibody anti- CCDC93 mouse monoclonal Santa Cruz Biotechnology #sc- 514600 IB (1:100) Antibody anti- alpha- tubulin mouse monoclonal DSHB #12G10, RRID:AB_1210456 IB (1:1000) Antibody HRP- conjugated anti- FLAG M2 mouse monoclonal Sigma- Aldrich #A8592, RRID:AB_439702 IB (1:1000) Antibody HRP- conjugated anti- HA rat monoclonal Roche #12013819001, RRID:AB_390917 IB (1:1000) Antibody HRP- conjugated anti- rabbit IgG goat polyclonal Sigma- Aldrich #A6154, RRID:AB_258284 IB (1:2000) Antibody HRP- conjugated anti- mouse IgG sheep polyclonal Sigma- Aldrich #A6782, RRID:AB_258315 IB (1:2000) Software, algorithm AlphaFold3 (AF3) PMID:38718835 Used for structural prediction Continued Cell lines and cell culture HeLa and 293T cells were obtained from ATCC and had been authenticated by the vendor. ..

Article Title: Pharmacochemical Studies of Synthesized Coumarin-Isoxazole-Pyridine Hybrids.
Article Snippet: .. Cell Culture HeLa (cervical cancer), HT-29 (colorectal cancer), and H1437 (lang adenocarcinoma) cell lines were obtained from American Type Culture Collection (ATCC) and cultured in Dulbecco’s Modified Eagle Medium (DMEM). ..

Article Title: Activated GTPase-based assays and kits for the diagnosis of sepsis and other infections
Article Snippet: .. Cell Culture HeLa and Vero E6 cells from ATCC were plated at 2×104 cells per well in a 48-well plate, then allowed to grow for ̃48 h to medium/low confluence in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% FBS, pen/strep, and L-glutamine in a 37° C. incubator with 5% CO2. ..

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